Abstract Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted Wooden Dolls mutagenesis.Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing.Herein, we introduce two strategies to improve the editing efficiency using proxima
Background Insomnia not only decreases the quality of life and working efficiency, but also relates to other deceases.Suitable exercises may be useful to improve sleep quality.However, comparative studies on the effects of different types of exercise on sleep outcomes are currently lacked.Objective To compare the effects of different exercise types
This study focuses on the sources of alkali and alkaline-earth elements based on the geochemistry of groundwater and surface water in Dschang concerning environmental and anthropogenic constraints.A comprehensive set of 50 samples from groundwater and surface water were analyzed by ICP-MS and processed by spatial interpolation in a GIS environment.
Mutations of WD repeat domain 62 (WDR62) lead to autosomal recessive primary microcephaly (MCPH), and down-regulation of WDR62 expression causes the loss of neural progenitor cells (NPCs).However, how WDR62 is regulated and hence controls neurogenesis and brain size remains elusive.Here, we demonstrate that mitogen-activated protein kinase kinase k